Gentamicin invasion assay.

<p>The ability of <i>S.</i> Typhimurium SL1344(p1C/1) to invade ESDCs was measured by the gentamicin assay described in Methods. ESDCs were exposed to either wild type <i>S</i>. Typhimurium or a <i>SipB</i> mutant for 30 minutes and then incubated with media containing 50 µg/ml of Gentamicin for 2 or 4 hours and then invading bacteria were enumerated.