Cessation of cell division <i>in vivo</i>. A–H)

<p>Epifluorescence images of HuNu and Ki-67 immunofluorescences in sections of transplanted animals, two months post-grafting. <b>A–D)</b> Low magnification images comparing the transplants of polyclonal hVM1-Bcl-X_L cells (A, B) and a representative clone (# 30; C, D). HuNu allows for the identification of all human cells in the rat section and Ki-67 labels cells engaged in the cell cycle. Note the difference in graft volume between the polyclonal cells and the clone, and that the transplant of hVM1-Bcl-X_L cells looks heavily stained for Ki-67. Scale bar in D, 1 mm, valid for A-D. CC, corpus callosum; LV, lateral ventricle. <b>E–H)</b> Higher magnification view of two representative clones, showing poor (clone # 28; E, F) and good (clone # 32; G, H) downregulation of Ki-67. Scale bar: 40 µm, valid for E-H. <b>I–J)</b> Details of neurons differentiated in the transplants of clone 32, immunostained for hNSE (I) or TH (J). Scale bar in J: 20 µm, valid for I, J.