<p>CD11b<sup>+</sup>Ly6C<sup>+</sup> or CD11b<sup>+</sup>Ly6C<sup>−</sup> DCs isolated from the BLNs of HDM-treated mice were transferred i.p. into naive recipients (n = 6 recipient mice per group). Recipients were challenged and sacrificed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053242#pone-0053242-g001" target="_blank">Figure 1</a>. A, Proliferation of BLN cells after <i>in vitro</i> HDM stimulation. B, ELISA measurement of IL-4, IL-5, IL-13, IL-17 and IFN-γ in the supernatant of HDM-stimulated BLN cells. C, Total and differential cell counts in BAL fluid.</p
<p>A) B6 mice were left untreated or injected i.v. with PKH67-labeled BALB/c apoptotic splenocytes. ...
<p>(A–D) Flow cytometry of CD4 versus IL-17 among total cells (A) and IL-17 versus IFNγ among gated ...
<p>4-get mice (BALB/c background) were inoculated i.t. with: saline or 50 μg HDM once (Panel A), sal...
<p>Lung CD11b<sup>−</sup>CD103<sup>+</sup> and CD11b<sup>−</sup>CD103<sup>−</sup> cells from HDM-tre...
<p>Lung DCs from saline- or HDM-treated mice (saline/or HDM/) were isolated and cultured in the pres...
<p>Naive mice were transferred i.t. with FACS-sorted lung CD11c<sup>+</sup> DCs from saline- or HDM-...
<p>A, Flow cytometric assessment of the expression levels of MHC-II, CD40, CD80, CD86, Sirp-α, FcεR-...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>A, Percentage of CD11b<sup>+</sup>Ly6C<sup>+</sup>, CD11b<sup>+</sup>Ly6C<sup>−</sup>, CD11b<sup>...
<p>(A) Specific populations of CD11b<sup>high</sup> DCs were sorted from the lungs of mice infected ...
<p>(A) Flow cytometry of CD11b versus I-A/I-E, CD11c versus CD11b, and CD45.2 versus CD11b in the br...
<p>The lymphocytes of the draining popliteal lymph nodes of 5 BALB/c or 5 CD11c<sup>cre</sup>IL-4Rα<...
<p>CC10-OVA.RAG<sup>-/-</sup> or B6.RAG<sup>-/-</sup> mice were given 1x10<sup>5</sup> OT-I activate...
<p>MOG-CD8+ or OVA-CD8+ T cells were transferred intravenously into naïve mice, followed by either n...
<p>(A) Spleen and ILNs from the recipient mice are shown. Photos are representative of the recipient...
<p>A) B6 mice were left untreated or injected i.v. with PKH67-labeled BALB/c apoptotic splenocytes. ...
<p>(A–D) Flow cytometry of CD4 versus IL-17 among total cells (A) and IL-17 versus IFNγ among gated ...
<p>4-get mice (BALB/c background) were inoculated i.t. with: saline or 50 μg HDM once (Panel A), sal...
<p>Lung CD11b<sup>−</sup>CD103<sup>+</sup> and CD11b<sup>−</sup>CD103<sup>−</sup> cells from HDM-tre...
<p>Lung DCs from saline- or HDM-treated mice (saline/or HDM/) were isolated and cultured in the pres...
<p>Naive mice were transferred i.t. with FACS-sorted lung CD11c<sup>+</sup> DCs from saline- or HDM-...
<p>A, Flow cytometric assessment of the expression levels of MHC-II, CD40, CD80, CD86, Sirp-α, FcεR-...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>A, Percentage of CD11b<sup>+</sup>Ly6C<sup>+</sup>, CD11b<sup>+</sup>Ly6C<sup>−</sup>, CD11b<sup>...
<p>(A) Specific populations of CD11b<sup>high</sup> DCs were sorted from the lungs of mice infected ...
<p>(A) Flow cytometry of CD11b versus I-A/I-E, CD11c versus CD11b, and CD45.2 versus CD11b in the br...
<p>The lymphocytes of the draining popliteal lymph nodes of 5 BALB/c or 5 CD11c<sup>cre</sup>IL-4Rα<...
<p>CC10-OVA.RAG<sup>-/-</sup> or B6.RAG<sup>-/-</sup> mice were given 1x10<sup>5</sup> OT-I activate...
<p>MOG-CD8+ or OVA-CD8+ T cells were transferred intravenously into naïve mice, followed by either n...
<p>(A) Spleen and ILNs from the recipient mice are shown. Photos are representative of the recipient...
<p>A) B6 mice were left untreated or injected i.v. with PKH67-labeled BALB/c apoptotic splenocytes. ...
<p>(A–D) Flow cytometry of CD4 versus IL-17 among total cells (A) and IL-17 versus IFNγ among gated ...
<p>4-get mice (BALB/c background) were inoculated i.t. with: saline or 50 μg HDM once (Panel A), sal...