Systemic administration of IL-10 diminishes SPX-induced fat accumulation, infiltration of macrophages, and pro-inflammatory responses in the pancreas.

<p>(<b>A</b>) Representative images of H&E staining, oil-red O staining, CD11c staining, and CD206 staining in intra-lobular areas in pancreas sections from each group. Scale bar = 100 µm. (<b>B−E</b>) TG content (<b>B</b>), M1/M2 ratios (<b>C</b>), content of pro- and anti-inflammatory cytokines (<b>D</b>), and IL-10/IL-1β ratios (<b>E</b>) in the pancreas of each group (<i>n</i> = 6). *<i>P</i><0.05 vs. the Standard-Sham group, ^#<i>P</i><0.05 vs. the HF-Sham group. Treatment groups: Standard-Sham, fed standard chow, given serum albumin, and sham-operated; HF-Sham, fed a HF diet, administered serum albumin, and sham-operated; HF-SPX, fed a HF diet, given serum albumin, and splenectomized; HF-SPX+IL-10, fed a HF diet, given IL-10, and splenectomized; Pair-fed, administered serum albumin, sham-operated, and fed the same amount of food as that consumed by the HF-SPX group.