Antibody inhibition of leukocyte migration by mAbs 130-6D and 131-2G.

<p>The inhibition of RSV A2 G protein – mediated leukocyte chemotaxis by anti-RSV G antibodies (130-6D and 131-2G) was determined as described in Materials and Methods. Antibody was included in the lower chamber of the chemotaxis chamber, along with purified RSV A2 G protein, at an equal concentration (1X) (22.5 µg/mL) to that of purified RSV G protein (22.5 µg/mL) up to a 50-fold concentration (50X) of mAb to purified RSV G protein. For combination mAb inhibition, 1X and 50X of each antibody were used. Each condition was assayed three times per experiment. Data is presented as an average percent inhibition of RSV A2 G protein mediated leukocyte chemotaxis ± standard deviation relative to the amount of chemotaxis induced by RSV A2 G protein alone. Asterisk indicates significant difference in inhibition (p<0.05) as determined by comparing to 131-2G (1X) or 130-6D (1X) mAb inhibition alone to combination of 131-2G and 130-6D mAbs (1X) or 130-6D (50X) mAb inhibition alone to combination of 131-2G and 130-6D mAbs (50X).