<p>Lane 1: Crude lysate sample before IPTG induction; Lane 2: Supernatant from inclusion body in induced cells; Lane 3: Unpurified refolded CST1 extract from inclusion body; Lane 4: Purified refolded CST1; Lane 5, Standard lumbrokinases; M: protein molecular marker.</p
<p>Upon IPTG induction, rhG-CSF was analyzed using a 4–12% reducing SDS-PAGE gel followed by Coomass...
<p>(A) 12% SDS-PAGE analysis of the samples collected from different steps of expression and purific...
<p>(A) Western analysis of transfectants with anti GFP. Lane 1 WCL of S1; Lane 2: WCL of VC [S1 (pXG...
<p>Lane 1: Crude lysate of <i>E. coli</i> BL21 transformed with empty plasmid; Lane 2: Uninduced sam...
<p>Columns: 1: whole lysate of cells harboring PMAL-C2x after IPTG induction; 2: whole lysate of cel...
<p>Lane 1: protein molecular standard; lane 2: negative control for rCfNOS (without induction); lane...
<p>Lane 1: protein molecular standard (kDa); lane 2: negative control for rCfC1qDC (without inductio...
<p>Lane 1: protein molecular standard; lane 2: negative control for rCfLec-1 (without induction); la...
<p>Lane M: protein molecular standard (kDa). Lane 1: negative control for rCfDDC (without induction)...
<p>(<b>A</b>) Reduced SDS-PAGE analysis of lysate samples prior to purification. Lanes 1–3 represent...
(A) Western blots display the protein levels of four proteins PKM, CLU, KNG1 and PFN1 in N- and CSD-...
<p>Arrows indicate the protein of interest. Other bands are the result of non-specific binding. The ...
<p>Blots were probed with anti-rCs1 monoclonal antibody (Lanes 1, 3, and 5) or S/P20 (Lanes 2, 4, an...
<p>A. Western blot of GFP and different GFP-labeled Htt fragments run on a SDS polyacrylamide gel. B...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>Upon IPTG induction, rhG-CSF was analyzed using a 4–12% reducing SDS-PAGE gel followed by Coomass...
<p>(A) 12% SDS-PAGE analysis of the samples collected from different steps of expression and purific...
<p>(A) Western analysis of transfectants with anti GFP. Lane 1 WCL of S1; Lane 2: WCL of VC [S1 (pXG...
<p>Lane 1: Crude lysate of <i>E. coli</i> BL21 transformed with empty plasmid; Lane 2: Uninduced sam...
<p>Columns: 1: whole lysate of cells harboring PMAL-C2x after IPTG induction; 2: whole lysate of cel...
<p>Lane 1: protein molecular standard; lane 2: negative control for rCfNOS (without induction); lane...
<p>Lane 1: protein molecular standard (kDa); lane 2: negative control for rCfC1qDC (without inductio...
<p>Lane 1: protein molecular standard; lane 2: negative control for rCfLec-1 (without induction); la...
<p>Lane M: protein molecular standard (kDa). Lane 1: negative control for rCfDDC (without induction)...
<p>(<b>A</b>) Reduced SDS-PAGE analysis of lysate samples prior to purification. Lanes 1–3 represent...
(A) Western blots display the protein levels of four proteins PKM, CLU, KNG1 and PFN1 in N- and CSD-...
<p>Arrows indicate the protein of interest. Other bands are the result of non-specific binding. The ...
<p>Blots were probed with anti-rCs1 monoclonal antibody (Lanes 1, 3, and 5) or S/P20 (Lanes 2, 4, an...
<p>A. Western blot of GFP and different GFP-labeled Htt fragments run on a SDS polyacrylamide gel. B...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>Upon IPTG induction, rhG-CSF was analyzed using a 4–12% reducing SDS-PAGE gel followed by Coomass...
<p>(A) 12% SDS-PAGE analysis of the samples collected from different steps of expression and purific...
<p>(A) Western analysis of transfectants with anti GFP. Lane 1 WCL of S1; Lane 2: WCL of VC [S1 (pXG...
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