Maturation of F508del protein at low temperature.

<p>Pieces of ileal mucosa (∼5 cm) isolated from WT or homozygous F508del CF mice were mounted in large aperture tissue holders and incubated at 26°C for 14 h under carbogen gassing. Subsequently, epithelial cells were harvested by high-frequency vibration and a crude membrane fraction was isolated and subjected to SDS-PAGE (20 µg protein/lane). Wild type (WT) and F508del <i>Cftr</i> was detected by Western blotting using the R3195 (1∶1000) antibody. A representative result of four different experiments is shown. Band intensities were estimated by digital scanning (see Methods), showing an increase of F508del CFTR band C to approximately 25% (+/−5 SEM, N = 4) of wild type levels.