Effects of cholecystokinin on plasma cholesterol and triglycerides in fasting mice.

<p>Wild-type and <i>LDLR^−/−</i> mice were injected with 50 ng/kg of [Thr28, Nle31]-cholecystokinin (CCK) via the tail vein. <b>A–C.</b> Blood samples were collected before (control, 0 min) and at 2 h after CCK treatment. Plasma triglycerides and total cholesterol (TC) and free cholesterol (FC) were measured colorimetrically. Esterified cholesterol (EC) was calculated as the difference between TC and FC. Values represent the mean ± SEM of 6 independent experiments. The difference between control and CCK treatment was analyzed by Student's paried <i>t</i>-test. * <i>P</i><0.05 compared to control. <b>D.</b> Blood samples were collected before (0 time) and at 30 and 120 min after CCK treatment. Plasma CCK levels were determined using an enzyme immunoassay kit. Values represent the mean ± SEM of 4 independent experiments. The differences among time points in wild-type and <i>LDLR^−/−</i> mice were analyzed by two-way ANOVA followed by Tukey post-hoc tests. * <i>P</i><0.05 compared to the same genotype mice before (0 min) and at 30 or 120 min after CCK treatment.