Sub-chronic dantrolene treatment rescues LTP on RyR inhibition in 3xTg-AD mice.

<p><i>(</i><b><i>A–B</i></b><i>)</i> Left, graphs show averaged time course of LTP from NonTg and 3xTg-AD mice that were given daily injections of 0.9% saline (<b><i>A</i></b>, n = 7 for NonTg, n = 7 for 3xTg-AD) or 10 mg/kg dantrolene (<b><i>B</i></b>, n = 10 for NonTg, n = 14 for 3xTg-AD) for 4 weeks. Insets (above) show representative fEPSP traces before (1) and after (2) tetanus from NonTg and 3xTg-AD mice. Bar graphs on right show averaged % change in post-tetanus responses relative to baseline from NonTg and 3xTg-AD mice. <i>(</i><b><i>C–D</i></b><i>)</i> Left, graphs show averaged time course of LTP from NonTg mice <i>(</i><b><i>C</i></b><i>)</i> injected with saline (n = 5) or dantrolene (n = 12) with bath application of 10 µM dantrolene, and 3xTg-AD mice <i>(</i><b><i>D</i></b><i>)</i> injected with saline (n = 8) or dantrolene (n = 7) with bath application of 10 µM dantrolene. Insets (above) show representative fEPSP pre-tetanus traces before (1) and after (2) bath application of 10 µM dantrolene and post-tetanus fEPSP traces (3) with 10 µM dantrolene. Bar graphs on right show averaged % change in post-tetanus responses relative to baseline in aCSF or dantrolene from NonTg and 3xTg-AD mice. Baseline fEPSPs were recorded for 20 min at 0.05 Hz before and for 60 min at 0.05 Hz after LTP induction. The arrow indicates the time of tetanus. * = significantly different after 10 µM dantrolene bath application, p<0.05, n denotes number of slices.