Time course of contactin-2 mRNA expression after spinal cord injury.

<p>(A) Quantitative PCR (qPCR) shows expression of contactin-2 mRNA in the spinal cord caudal to the lesion site at four time points after SCI. Significant upregulation was observed at 6 and 11 days after SCI compared to the sham injury group (*p<0.05, one-way ANOVA with Tukey’s post-hoc test; n = 8 fish/group). Values represent means+SEM. (B) In situ hybridization detection of contactin-2 mRNA in longitudinal sections of the spinal cord 3 mm caudal to the lesion site at 4 and 12 hrs, and at 6 and 11 days after SCI (n = 3 fish/group). Numbers of positive cells were increased along the central canal at 6 and 11 days after SCI compared to the sham injury group at 11 days. No signal was detected in sections incubated with a sense probe. * indicates the central canal. Scale bar, 400 µm.