KLF8 directly binds to C/EBPα and PPARγ2 promoter.

<p>(A) EMSA experiment using KLF binding sites of β-globin (positive control), C/EBPα, or PPARγ2 promoter as probes. A FLAG-tagged KLF8 protein was in vitro translated using TNT T7 quick master mix. (B) EMSA experiment using wild type or mutated probe of the C/EBPα promoter. FLAG-tagged C/EBPβ-LAP or KLF8 protein was in vitro translated using TNT T7 quick master mix. (C) Western blot analysis of the in vitro translated proteins. n.s, non-specific. (D) ChIP was performed on 3T3-L1 cell chromatin at the indicated time points after induction of differentiation, using control IgG, anti-C/EBPβ, or anti-KLF8 antibody. The immunoprecipitated DNA was used as a PCR template to detect the PPARγ or C/EBPα promoter regions.