Expression and localization of HIV Gag-GFP fusion proteins.

<p>(A) Live cells (SEY6210 or JPY69 for ) expressing the indicated form of Gag-GFP were visualized by fluorescence microscopy. Scale bar for each image represents 4 . (B) Yeast cells expressing either WT Gag-GFP or were analyzed for their ability to incorporate these chimeras into VLPs. Whole cell extracts (W.C.) and the VLP fractions were subjected to SDS-PAGE and western blotting with anti-GFP antibody to allow visualization. (C) Representative images of fluorescence recovery after photobleaching performed on wild type cells expressing Gag-GFP (pre-bleach, first image captured post-bleach, and 25 minute recovery). The red box highlights a punctum that was subjected to bleaching while the red arrows highlight Gag-GFP puncta that were not. (D) Quantification of fluorescence recovery after photobleaching (at one frame per minute) for wild type and cells expressing wild type Gag-GFP.