ATRA induces the expression and <i>N</i>-glycan modification of ICAM-1.

<p>A, SW480 cells were treated with 0, 5, 10, 25 and 50 µM ATRA for 36 h or with 25 µM ATRA for 0, 12, 24 and 36 h. The expression of ICAM-1 was analyzed by real-time RT-PCR (n = 3) and Western blot. Equal loading was verified by detection of GAPDH. B, HUVEC cells were treated with 25 or 50 µM ATRA for 0, 24 and 36 h. The expression of ICAM-1 was analyzed by real-time RT-PCR (n = 3) and Western blot. C, U937 and WISH cells were treated with either 10 ng/ml IFN-γ or 25 µM ATRA or both. The expression of ICAM-1 was analyzed by Western blot. D, WISH cells were treated with 25 µM ATRA. The cell lysates were immunoprecipitated by the antibody against ICAM-1 and then treated with PNGase F. The digested samples were blotted and detected by Western blot.