Histological assessment of <i>L. sigmodontis</i> and <i>M. tuberculosis</i> infection at the 20 week timepoint.

<p><i>A</i>, lung with <i>M. tuberculosis</i> (MTB) granulomas obtained from a co-infected animal 9 weeks post MTB challenge (the 20 week timepoint). <i>B</i>, Lung granuloma (red arrow) with central necrosis (green arrow) observed in the lung of a cotton rat infected with MTB (H&E, 40×). <i>C</i>; Acid-fast stain of MTB bacteria in the lung (100×). <i>D</i>, <i>L. sigmodontis</i> microfilaria in peripheral blood (Eosin-Y Azure A Methylene Blue, 100×). <i>E</i>, H&E stained cross-section of lung tissue that shows a <i>L. sigmodontis</i> adult worm in the pleural space adjacent to the lung (40×).