Effect of anti-leishmanial drugs on ATP synthesis and ΔΨm in resistant <i>L. donovani</i> promastigote lines.

<p>(<b>A</b>) ATP levels were measured using CellTiter-Glo. (<b>B</b>) ΔΨm was measured by determining the accumulation of Rh123 (0.5 µM) in WT and resistant lines. In both cases, promastigote log-phase cultures were left untreated (controls, black columns) or exposed to 0.2 µM AmB (white columns), 25 µM MLF (oblique lines columns) for 3 h, 2 mM Sb^III (gray columns) for 8 h, or 10 µM FCCP as a depolarization control (light gray columns) for 10 min. Measurements are expressed in arbitrary luminescence (panel A) or fluorescence (panel B) units ± SD from three independent experiments and show significant differences (^a: <i>p</i><0.05; ^b: <i>p</i><0.01; ^c: <i>p</i><0.005; ^d: <i>p</i><0.001) when comparing the corresponding control values for each line with itself after treatment. A comparison of the different untreated lines with untreated WT lines also shows significant differences (*: <i>p</i><0.05; **: <i>p</i><0.005; ***: <i>p</i><0.001).