Intracellular protein levels of IL-2, IL-17 and IFN-γ are enhanced by concomitant treatment with natalizumab.

<p>(A, B) PBMC were stimulated by SEB or anti-CD3/28 without or in the presence of natalizumab for 24 h. After surface staining for CD4 and intracellular staining for IL-2, cells were analyzed by FACS. (A) Representative dot blots are given. (B) Single values of samples from n = 11 (SEB) and n = 17 (anti-CD3/28) donors are shown. (C, D) Isolated CD4⁺ T cells were stimulated with T/I (n = 9) or ionomycin (n = 10) for 8 h. Intracellular FACS stainings for IL-2, IL-17, and IFN-γ were performed, and the differences between cultures with and without natalizumab were calculated. Two-tailed Wilcoxon matched-pairs signed rank tests were performed for statistical analysis in (B, C, and D).