The effect of BSE and CSE on TIMP-1, MMP-1 and IL-8 expression in human SAE cells.

<p>(A) TIMP-1 levels were measured from the media of SAE cells treated with 2% or 5% of BSE or CSE for 24 hours via ELISA. N = 6 per group. Data is reported as mean ± standard error of measurement. (B) Endotoxin levels were measured in phosphate buffered saline (PBS) and biomass smoke extract (BSE) and cigarette smoke extract (CSE) before and after endotoxin removal. N = 4 in each group. Data is reported as mean ± standard error of measurement. (C) MMP-1 (left panel) and IL-8 (right panel) levels were measured from the media of SAE cells treated for 24 hours with 2% BSE, CSE or 5% BSE, CSE with and without endotoxin removal and 1 µg/ml LPS for 24 hours via ELISA. N = 6 per group. Data is reported as mean ± standard error of measurement. *Indicates endotoxin removal.