Green tea polyphenols decrease cell viability and induces apoptosis in LNCaPshV and LNCaPshp53 cells.

<p>[<b>A</b>] Cells were exposed to 20–80 µg/ml concentration of GTP for 24 h, and viability of the cells was determined by the MTT assay. Cell viabilities are depicted as percentages; vehicle-treated cells were regarded as 100% viable. The bars represent mean±SD of at least two independent experiments each performed in duplicate, **<i>p</i><0.001 represents significant differences as compared to control group without GTP treatment. [<b>B</b>] Cell were treated with 40 µg/ml GTP for 24, 48, 72 and 96 h and distribution of cells were recorded in different stages of cell cycle analyzed using FACS analysis. [<b>C</b>] Cells were treated with 40 and 80 µg/ml GTP for 96 h and the number of cells undergoing apoptosis were determined by measuring cell population in sub G1 phase of the cell cycle. The bars represent mean±SD of at least two independent experiments each performed in duplicate, **<i>p</i><0.001 represents significant differences as compared to control group without GTP treatment. [<b>D</b>] Light microscopic images of LNCaPshV and LNCaPshp53 cells treated with 40 and 80 µg/ml GTP for 96 h. GTP treatment exhibits morphological changes consistent with apoptosis in both these cells. [<b>E</b>] DNA fragmentation assay. The cells were treated with 40 µg/ml concentration of GTP for 48 h, collected for DNA isolation and subjected to agarose gel electrophoresis, followed by visualization of bands under UV light. The details are described in the materials and methods section.