Mitochondrial fragmentation following hypoxia-reoxygenation is ameliorated by inactivation of MARCH5 or Drp1.

<p>(A) Differentiated RGC5 cells expressing MARCH5^YFP, MARCH5^H43W-YFP, Drp1^YFP or Drp1^K38A–YFP were cultured in the presence of low oxygen (1%) for 24 hours followed by normoxia for 2 hours. Mitochondrial fragmentation was analyzed following cytochrome <i>c</i> staining in three independent experiments (>200 cell counted/condition). Error bars represent SEM, p-Values for Student's t-test are marked with * (p<0.05) or ** (p<0.01). (B) RGC5 cells expressing MARCH5 or MARCH5^H43W and PAGFP were stressed using hypoxia-reoxygenation and mitochondrial interconnectivity was assessed by measuring PAGFP diffusion following photoactivation. Analyzed were 20 cells/condition with error bars representing SEM and ** marking p<0.01 and n.s. marking p>0.05 (Student's t-test).