Relative comparative <i>wt</i> and <i>spect (</i>−<i>)</i> parasite DNA load in spleen and liver of immunized mice.

<p>To compare relative load of <i>wt</i> and <i>spect (</i>−<i>)</i> parasite DNA in liver and spleen, BALB/c (3 mice per group) were immunized iv on tail with 1×10⁵ iSPZ (<i>wt</i> or <i>spect (</i>−<i>)</i>) in 500 µl of RPMI. Two hours later, a real-time PCR was performed following extraction of respective parasite RNA. Every sample was done in duplicate. To avoid any contamination by eventual parasite from blood, the liver and spleen were perfused with PBS before performing the RT-PCR. Figures <b>a</b> and <b>b</b> represent <i>wt</i> and <i>spect (</i>−<i>)</i> parasite DNA load, respectively, in the liver and spleen 2 h after iSPZ injection (iv). Naïve mice (receiving only 500 µl iv of DMEM) were used as a control.