Time-course effects of 1,25(OH)₂D₃ on adipogenic marker expression.

<p>Human preadipocytes were differentiated in the absence or presence of 1,25(OH)₂D₃ (10⁻⁸ M, added continuously throughout). Expression levels of adipogenic markers [C/EBPβ (A), C/EBPα (B), PPARγ (C), and LPL (D)] were measured before (0′) and at indicated time points during differentiation. Data are presented as % of vehicle control after differentiation (d10–12; d10+) in each experiment. *, p<0.05, vehicle control vs. 1,25(OH)₂D₃ treatment, n = 4. E. Representative FABP4 and VDR blots from 3 independent experiments are presented.