Effect of IL-6 deficiency on the accumulation of bone marrow-derived fibroblast precursors in the kidney after UUO.

<p><b>A.</b> Representative photomicrographs of kidney sections from WT and IL-6 KO mice 5 days after UUO stained for CD11b (red), procollagen I (green), and DAPI (blue). <b>B.</b> Quantitative analysis of CD11b⁺ and procollagen I⁺ fibroblasts in the kidney of WT and IL-6 KO mice 5 days after UUO. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT-UUO, and ⁺⁺<i>P</i><0.01 vs KO-UUO. n = 4 per group. <b>C.</b> Representative photomicrographs of kidney sections from WT and IL-6 KO mice 1 week after UUO stained for CD11b (red), procollagen I (green), and DAPI (blue). <b>D.</b> Quantitative analysis of CD11b⁺ and procollagen I⁺ fibroblasts in the kidney of WT and IL-6 KO mice 1 week after UUO. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT-UUO, and ⁺⁺<i>P</i><0.01 vs KO-UUO. n = 5 per group. <b>E.</b> Representative cytometric diagrams showing the effect of IL-6 deficiency on the accumulation of CD11b and collagen I dual positive fibroblasts in the kidney of WT and IL-6 KO mice 1 week after UUO. <b>F.</b> Quantitative analysis of CD11b and collagen I dual positive fibroblasts in the kidney of WT and IL-6 KO mice 1 week after UUO. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT UUO, and⁺⁺<i>P</i><0.01 vs KO UUO. n = 3 per group.