Melatonin attenuates APAP-induced hepatic RIP1 activation and JNK phosphorylation.

<p>Mice were treated as Materials and Methods. Liver samples were collected at 1 h after APAP administration. (A) Hepatic RIP1 was detected by immunoblots. (B) All mice except controls were i.p. injected with APAP (300 mg/kg). In melatonin+APAP group, mice were i.p. injected with different doses of melatonin (1.25, 5, 20 mg/kg) 30 min before APAP (300 mg/kg, i.p.). Liver samples were collected at 4 h after APAP administration. Hepatic phosphorylated JNK was detected by immunoblots. All experiments were repeated for four times. Quantitative analyses of scanning densitometry on four different samples were performed. All data were expressed as means±SEM (n = 4). **<i>P</i><0.01 as compared with the control. ‡‡ <i>P</i><0.01 as compared with APAP group.