Effect of IL-6 deficiency on profibrotic molecule expression.

<p><b>A.</b> IL-6 deficiency does not affect CXCL16 gene expression. The mRNA levels of CXCL16 in the kidneys of WT and IL-6 KO mice were determined by real-time RT-PCR. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT UUO, and ⁺<i>P</i><0.05 vs KO UUO. n = 3–4 per group. <b>B.</b> IL-6 deficiency does not influence TGF-β1 gene expression. The mRNA levels of TGF-β1 in the kidneys of WT and IL-6 KO mice were determined by real-time RT-PCR. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT UUO, and ⁺<i>P</i><0.05 vs KO UUO. n = 3–4 per group. <b>C.</b> IL-6 deficiency does not influence IL-4 gene expression. The mRNA levels of IL-4 in the kidneys of WT and IL-6 KO mice were determined by real-time RT-PCR. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT UUO, and ⁺<i>P</i><0.05 vs KO UUO. n = 3–4 per group. <b>D.</b> IL-6 deficiency does not influence IL-13 gene expression. The mRNA levels of IL-13 in the kidneys of WT and IL-6 KO mice were determined by real-time RT-PCR. ** <i>P</i><0.01 vs WT control, ^#<i>P</i>>0.05 vs WT UUO, and ⁺<i>P</i><0.05 vs KO UUO. n = 3–4 per group.