Effects of unadjuvanted and GPI-0100-adjuvanted mucosal influenza vaccine on lung virus titers.

<p>Mice were immunized on day 0 and on day 20 with 1 µg A/PR/8 subunit vaccine alone or adjuvanted with the indicated doses of GPI-0100. The control mice received HBS buffer. The immunizations were given via intramuscular (i.m.), intranasal (i.n.) or intrapulmonary (pul.) route. 2 weeks after the second immunization, mice were infected with live A/PR/8 virus. Lung samples were collected 3 days later upon termination. Virus titer is expressed as the 10log virus titer per gram of lung tissue for individual mice. The black line represents the geometric mean virus titer per group. One mouse from the HBS control group was sacrificed before the challenge due to abnormal tissue growth with unknown reason. Levels of significance are depicted as follows: *p<0.05, **p<0.01 and ***p<0.005.