Lentiviral vectors used to target transgene expression to macrophage-derived cells.

<p>(<b>a</b>) Schematic diagram of the lentivirus vectors used to target cells of immune lineage. (<b>b</b>) miR-142 expression levels in Huh7, 293T and NR8383 cells detected by RT-qPCR. Three independent experiments were performed in duplicate. * Statistically significant differences <i>P</i> = 0.043 (two-tailed, unpaired Student’s <i>t</i>-test). (<b>c</b>) <b>&</b> (<b>d</b>) Huh7, 293T and NR8383 cells were transduced with two different lentiviral vectors carrying the TetR-KRAB sequence follow by 4 copies of miR-142 target (c) or miR-122 (d). After 10 days of culture with or without doxycycline, transduced cells were analyzed for GFP expression by FACS. Results are expressed as a percentage of mean fluorescence intensity (MFI). In TetR-Krab system, the expression of GFP is optimal in the presence of doxycycline, corresponding to 100% of the MFI. The raw data corresponding to 100% are indicated above each histogram bar.Data shown are mean and error bars indicate the SD of 3 independent experiments performed in triplicate.