IR mRNA and protein levels in the OB are not modulated by feeding states.

<p><i>(A)</i> Bar graphs represent the relative abundance of IR mRNA in the OB of satiated (gray bar; n = 6) and fasted (white bar; n = 6) rats as determined by semi-quantitative RT-PCR and using β-actin mRNA for normalization (mean ± SEM). Representative Western blots <i>(B)</i> and quantitative densitometric histograms <i>(C)</i> of the IR protein levels in the OB of satiated (gray bar, S; n = 4) and fasted rats (white bar, F; n = 4) (mean ± SEM). Bar graphs represent ratios of the IR subunits (IRα, and IRβ) protein levels after normalization against actin. The means were not statistically different (Mann-Whitney test, p>0.05).