CFTR protein expression in Calu-3 cells in the presence of cycloheximide with or without dexamethasone.

<p>Calu-3 cells were treated (<b>A</b>) with (+) or (<b>B</b>) without (−) 10 nM Dx for 24 h, and then incubated in the presence of 100 µg/ml cycloheximide (CHX) for the indicated times (0.5, 1, 2, 4, and 8 h). In the graphs, CFTR protein expression is represented by the densitometric values of each blot using GAPDH as a housekeeper protein. For CHX+Dx, the protein expression was normalized by the group treated with dexamethasone for 24 h and for CHX the normalization was related to the C group. *Significantly different from the respective 24-h group; <i>p</i><0.05, <i>n</i> = 4.