Immunoprecipitation assay for CFTR protein followed by Western blot for HSP90 or HSP70 in Calu-3 cells.

<p>Cells were treated with (Dx) or without (C group) dexamethasone or with mifepristone (Dx+Mif) for 24 h and total cell lysate. (<b>A</b>) Immunoprecipitation with CFTR Ab and subsequent Western blot for HSP90 or HSP70. The graphs represent the densitometric values of the bands shown in the blots for (<b>B</b>) CFTR, (<b>C</b>), HSP90, and (<b>D</b>) HSP70. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047405#s3" target="_blank">Results</a> are presented as the average and SE. <i>n</i> = 4, <i>p</i><0.05.