Effect of shRNA expressing lentivirus against CB1 on CB1 expression in primary hepatic stellate cells.

<p>(<b>A</b>) The statistical results of CB1 mRNA expression in primary hepatic stellate cells (HSCs) transfected with 4 pairs of shRNA expressing lentivirus named KD1, KD2, KD3 and KD4 by RT-PCR detection. The pGCSIL/U6 mock vector named Con was used as a negative control. KD4 displayed the highest gene-silencing efficacy among all the 4 pairs of lentivirus. Relative expression levels of mRNA were normalized against those of GAPDH mRNA. (<b>B</b>) The CB1 protein expression in HSCs transfected with 4 pairs of shRNA expressing lentivirus and the negative control lentivirus, respectively. The results demonstrated that KD4 deduced CB1 mRNA expression by about 75%, and protein expression by about 80%, which had the highest gene-silencing efficacy among all the four pairs of lentivirus vectors.