Selective α7-nAChR agonist, PNU-282987, enhances insulin sensitivity in AMPKα2^−/− mice.

<p>(A) Genotype characterization by PCR analysis of tail DNA from offspring derived from heterozygous intercrosses. Expected fragment sizes of the wild-type mice (+/+; 200 bp), heterozygous knockout mice (+/−, 200 bp and 600 bp) and homozygous knockout mice (−/−; 600 bp) are shown. (B) Weight gain in mice during PNU-282987 treatment. At the end of treatment, (C) HOMA-IR, (D) insulin tolerance test (ITT) and (E, F) glucose tolerance test (GTT) were performed. ITT was performed in mice 6 h after food removal with insulin challenge at 0.55 IU/kg of body weight (i.p.). GTT was performed in overnight fasted mice with glucose challenge at 2.0 g/kg of body weight (i.p.). Data are means ± SE (n = 5–6). ^#<i>P</i><0.05, ^##<i>P</i><0.01 <i>vs</i> saline treatment.