C/EBPβ is involved in ER stress in MSC.

<p>A, C3H10T1/2 cells were transfected with 1 µg of C/EBPβ versus empty vector. 48 hours after transfection, RNA analysis was performed via real time PCR (n = 5). B, C3H10T1/2 cells transiently transfected with C/EBPβ versus empty vector at indicated doses and 48 hours after transfection, protein analysis was performed. A representative blot is shown, n = 3. C, Densitometry for (B). D, Immunoblot of C3H10T1/2 cells transfected with siRNA to C/EBPβ or control siRNA shown at 72 hours after transfection. Tunicamycin at 1 µg/ml was added for the final 6 hours. E, ChIP-seq analysis of C/EBPβ, β-catenin, TCF4 binding at the mouse <i>CHOP</i> and <i>BiP</i> gene loci. MC3T3 cells were treated for 3 hours with vehicle or 50 mM LiCl prior to ChIP-seq assay. The genomic interval on the indicated mouse chromosome and the location of the <i>CHOP</i> or <i>BiP</i> transcription unit including the direction of transcription (arrow) is shown at the top. Tracks indicate tag densities (normalized to 10⁷ reads) for vehicle or LiCl treated β-catenin or TCF4 binding. Note the scale for peak height is different for each track to highlight peak activities.