High frequencies of DN αβ T-cells are associated with TB severity.

<p>Representative contour plots showing the gate strategy used for the analysis of CD4 (middle left), CD8 (middle center), DN (middle right) αβ-T cells and the expression of CD69 (upper panels) and HLA-DR (lower panels) on DN αβ-T cells (A). Percentages of CD4⁺ (left panels), CD8⁺ (middle panels) and DN (right panels) αβ T-cells in healthy donors (HD, open symbols), TB (total TB, black symbols), nsTB (non-severe TB, light gray symbols) and sTB patients (severe TB, dark gray) were measured before treatment (B). The percentage of CD69 (C) and HLA-DR (D) expression within CD4⁺ (left panels), CD8⁺ (middle panels) and DN (right panels) αβ T-cells in HD, TB, nsTB and sTB patients were analyzed ex vivo. The boxes represent the means.