Mechanical strain ameliorates tunicamycin-induced ER stress in MSC.

<p>A, mdMSC were cultured in the presence of tunicamycin (TM) at indicated doses for 6 hours and analyzed via immunoblot for C/EBPβ, BiP and CHOP. B, as in (A) mdMSC cultured in the presence of tunicamycin 1 µg/ml for 6 hours and analyzed via conventional PCR for XBP1. C, C3H10T1/2 cells were seeded on the same day and treated with mechanical strain for the indicated number of days. Tunicamycin (TM) was added for the final 6 hours prior to protein analysis (representative blot, n = 3).