Subcellular localization of CUL3 and BPM1 proteins in the tobacco BY2 cells.

<p>CUL3 and BPM1 overlap in the cytoplasm but not in the nucleolus (A–D). After proteasome inhibition with 50 µM MG132, CUL3 and BPM1 accumulate in the cytoplasm which implies their cytoplasmic proteosomal degradation (E–H). Images shown are bright field (BF), Egfp and RFP fluorescence from CUL3 and BPM1 respectively, and a merged image. Arrowheads indicate cytoplasmic agglomerates where both proteins co-localize. Arrows indicate agglomerates of a particular protein. Bars are 50 µm.