Diseased TAK1^ΔM mice developed myelomonocytic leukemia.

<p>(<b>A</b>) Peripheral blood smears (<i>left panels</i>) and bone marrow smears (<i>right panels</i>) from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with Wright-Giemsa (WG) and H&E, respectively. Note numerous monocytes, some with azurophilic granules (<i>arrows</i>) in the peripheral blood and increased blasts in bone marrow aspirate smears of TAK1^ΔM mice. Also shown for the BM smear is the percentage of a manual count of blasts and monocytes (Mo). (<b>B</b>) Blood and bone marrow smears from diseased TAK1^ΔM mice were stained with Wright-Giemsa (WG) and non-specific esterase (NSE). Note the increased number of blasts in the peripheral blood film and sheets of blasts in the bone marrow aspirate smear (<i>arrows</i>). A subset of blasts was positive for NSE (<i>inset and lower panel</i>). (<b>C</b>) Bone marrow biopsies from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with H&E. Note the increased number of immature cells (<i>dotted circle</i>) in the TAK1^ΔM mice. Magnification: left panels, 200x; right panels, 400x. (<b>D–E</b>) Tissue sections of spleens from 24-week-old TAK1^F/+ and TAK1^ΔM mice were stained with H&E (<b>D</b>) and NASD (<b>E</b>, <i>left panel</i>) and immunostained with an anti-Mac-3 antibody (<b>E</b>, <i>right panel</i>).