Comparative expression patterns for <i>CCNA2, CCND1, CCND2, CCND3</i> and <i>CCNE2</i> mRNAs in normal and cancerous ovaries from laying hens.

<p>RT-PCR and quantitative RT-PCR analysis were conducted using cDNA templates from normal and cancerous ovaries of laying hens using chicken <i>CCNA2, CCND1, CCND2, CCND3</i> and <i>CCNE2</i> and chicken <i>GAPDH</i> specific primers (left panel). These experiments were conducted in triplicate and values normalized to those for GAPDH. <i>In situ</i> hybridization analysis indicates cell specific expression patterns for <i>CCNA2, CCND1, CCND2, CCND3</i> and <i>CCNE2</i> mRNAs in both normal and cancerous ovaries from laying hens (right panel). See <i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051592#s4" target="_blank">Materials and Methods</a></i> for complete description.