Effect of pretreated ES on established <i>P.aeruginosa</i>

<p><b>biofilm.</b> (a) ES from different CFU of <i>P. aeruginosa</i> fed sterile larva, then added to the well which contained <i>P.aeruginosa</i> biofilm, 24 h incubation, biofilms were exposed to a 1% crystal violet solution, and the amount of biofilm was measured by its A590. (b) Different amounts of ES from 1×10⁶ CFU of <i>P. aeruginosa</i> fed sterile larva were added to the well which contained <i>P.aeruginosa</i> biofilm, 24 h incubation, biofilms were exposed to a 1% crystal violet solution, and the amount of biofilm was measured by its A590. For 30 µg on, all values are significantly (<i>p<0.05</i>) different from these for biofilms without pretreated.(<b>--</b> nonpretreated group, <b>-</b>▪<b>-</b> pretreated with 10⁶ cfu/ml) (c) Light microscopic and scanning electron microscopic graphs of <i>P.aeruginosa</i> biofilm with or without infected ES. (c-1) Light microscopic graph of <i>P.aeruginosa</i> biofilm; (c-2) Light microscopic graph of <i>P.aeruginosa</i> biofilm which treated with infected ES ; (c-3) Scanning electron microscopic graphs of <i>P.aeruginosa</i> biofilm ;(c-4) Scanning electron microscopic graphs of <i>P.aeruginosa</i> biofilm which treated with infected ES.