α₂M provides signals necessary for T cell priming.

<p>a) RAW264.7 cells were pulsed with α₂M, LPS or PBS. The β-chain of CD91/LRP-1 was immunoprecipitated and probed with α-pTyr antibodies. b) Splenic dendritic cells were pulsed with α₂M (left panel) or PBS (right panel) for 20 h. Supernatants from either culture were incubated with cytokine profiler arrays as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050365#s2" target="_blank">Methods</a> and developed. Signals higher (blue boxes) or lower (red boxes) than background (PBS pulsed cells) are highlighted. Standardization signals on each blot (with+signs in Black boxes) are used to normalize signals to each other. c) Splenic dendritic cells were pulsed with α₂M or LPS in the presence or absence of the NF-κB inhibitor cardamonin for 20 h. Supernatants were analyzed by ELISA for TNF-α. Error bars represent standard deviation of duplicates.