miR-34a targets signaling pathways in human dental papilla cells.

<p>(A–E) Protein signals of predicted targets of miR-34a were detected by immunofluorescence in human dental papilla cells. (F) mRNA level of <i>NOTCH1</i> and <i>BMP7</i> were upregulated by inhibitor transfection. <i>LEF1</i>, <i>FGF2</i> and <i>GLI2</i> was not affected. (G) In contrast, mRNA level of <i>NOTCH1</i> and <i>BMP7</i> were downregulated by mimic transfection. <i>LEF1</i>, <i>FGF2</i> and <i>GLI2</i> was not affected. (H) Western blotting results indicated that protein level of these targets were regulated by either miR-34a mimic or inhibitor transfection. (*: <i>p</i><0.05 as determined by one-way ANOVA test followed by the post-hoc Tukey's test. Scale bar: 20 µm).