Anellovirus DNA was more prevalent in specimens from febrile children by high-throughput sequencing.

<p><b>A–B</b>. High-throughput sequencing analysis of plasma (<b>A</b>) and NP (<b>B</b>) specimens showed that a higher percentage of specimens from febrile children contained anellovirus DNA compared to specimens from afebrile children (plasma, p = 0.034; NP, p = 0.002). <b>C</b>. The trend was the same, but not statistically significant at the patient level of analysis. <b>D–E</b>. Afebrile and febrile patients had a similar median number of anellovirus sequence reads in their plasma (10.2 vs. 15.6) (<b>D</b>) and NP (2.9 vs. 1.7) (<b>E</b>) specimens. <b>F</b>. Anellovirus sequences detected by high-throughput sequencing were more prevalent in plasma compared to NP specimens (p<0.0001; Chi-squared test).