Influence of lactic acid on the induction of pCREB and influence of forskolin on CFTR phosphorylation in L6 cultured myocytes.

<p>A: sample gel showing the bands for pCREB, CREB and β-actin, which served as the internal control. Band density for pCREB (B) or total CREB (C) was expressed as a ratio to β-actin. Values are the mean ± SEM of 3 tests. *, significantly different from the value in the absence of lactic acid in 1-way ANOVA followed by the Bonferroni post-hoc t-test. D: sample autoradiograph showing the change in CFTR phosphorylation in response to forskolin (20 µM), forskolin with KT5720, or forskolin plus dibutyryl-cAMP (200 µM) and IBMX (1 mM). E: densitometry analysis of CFTR phosphorylation under the same conditions.