HIFU exposure setup.

<p>Our in-house built HIFU apparatus consisting of a motorized transducer, capable of movement in the x, y, and z planes, and a holder for the Opticell™ chamber submerged in a tank of degassed 37°C water. The walls of the tank and the area above the transducer and Opticell™ chamber were lined with an absorptive material, to prevent reflection of the acoustic beam and resulting standing wave formation.