Autophagy protects against oxaliplatin-induced cell death.

<p>(A and B) Caco-2 cells were treated with 100 nM rapamycin, 5 mM 3-MA, 10 nM Baf A1, 10 µM oxaliplatin, or the indicated combinations for 24 or 48 hours. The percentage of dead cells was determined using the MTT assay or cell death assay. (C) Detection of the inhibition efficiency of siRNAs against ATG5 and Beclin1. Caco-2 cells were transfected with siRNAs targeting ATG5 and Beclin1 (100 nM each) for 24 hrs, and the protein levels of the two targets were evaluated by western blot. (D and E) MTT and cell death assays measuring the oxaliplatin-treated cell death ratio after transfection with siRNAs against ATG5 and Beclin1. AGS cells were transfected with Beclin1, ATG5 or a control siRNA at 100 nM for 24 hrs followed by treatment with oxaliplatin (10 µM). The data shown are the means and SD of at least three independent experiments. *, <i>P</i><0.05.