Induction of an aggregation phenotype with strong biofilm-forming capabilities.

<p>A.) Percentage of continuous culture isolates with the aggregator phenotype from each treatment category. Isolates were tested from the 0 (white), 48 (gray), and 96 (black) hrs timepoints. B.) Biofilm-forming capability of continuous culture isolates as measured by crystal violet assay. PS80 is the ancestral strain. Aggregator and non-aggregator isolates were randomly selected from isolates from the GEN- and dual-treated continuous cultures. C.) The range of aggregator phenotypes seen when isolates were grown in TSB in 24-well plates. The starred well is an example of the parental, non-aggregator phenotype.