CaMKIV mediates affective, but not physical nicotine withdrawal signs. A.

<p>Nicotine-infused CaMKIV +/+ mice show significant anxiety-like behavior after mecamylamine (2 mg/kg, s.c. at day 15) treatment in chronically infused nicotine mice (36 mg/kg/day for 14 days). Withdrawal-induced anxiety-like behaviors are reduced in nicotine-infused CaMKIV −/− mice. The y axis represents the time spent in the open arms of the plus maze in seconds. <b>B</b>. Somatic nicotine withdrawal signs are not significantly attenuated in nicotine-infused CaMKIV −/− mice compared to +/+ counterparts. The y-axis represents the average number of somatic signs observed during the 20 min session. <b>C.</b> The nicotine withdrawal-induced hyperalgesia response is observed similarly in CaMKIV +/+ and −/− mice. The y-axis represents the average hot plate latency in seconds. Each point represents the mean ± SEM of 4–6 mice per group. *denotes p<0.05 vs. vehicle groups. **denotes p<0.05 vs. vehicle and CaMKIV −/− nic/mec group. Veh, vehicle; mec, mecamylamine; nic, nicotine.