Kindlin-1 and Kindlin-2 oppositely regulate lung cancer cell migration and invasion.

<p>a. Expression of Kindlin-1 and Kindlin-2 in the secondary <i>de novo</i> SCC from an invasive SCC patient. A. Kindlin-1 expression in the <i>de novo</i> SCC. B. Kindlin-2 expression in the <i>de novo</i> SCC from the same patient as in A. b. Left panel: Differential expression of Kindlin-1, Kindlin-2, E-cadherin and Vimentin in various lung cancer cell lines controlled by actin for loading. Right panel: Quantification of the relative protein expression levels. c. Left panel: Stable expression of Kindlin-1 in H1299 cells. Indicated are mixed stable clones that express Flag-Kindlin-1. Right panel: Quantification of the relative protein levels for Kindlin-1 regulated N-cadherin and Vimentin. d. Kindlin-1 and Kindlin-2 differentially regulate migration in H1299 cells. Displayed is the quantification and plot as mean ± SEM from three independent experiments. Statistical analyses between different cell groups were examined by Student's <i>t</i> test. ** represents for <i>p</i><0.01. e. Kindlin-1 and Kindlin-2 differentially regulate the mRNA levels of MMPs analyzed by qPCR in H1299 cells stably expressing Kindlin-1 and Kindlin-2. Insert shows expression of Kindlin-1 and Kindlin-2 in the stable mixed clones applied in qPCR. f. Left panels: Expression of Kindlin-1 downregulates N-cadherin and Knockdown of Kindlin-2 downregulates N-cadherin. Right panels: Quantification of the relative protein levels for Kindlin-1- and Kindlin-2-regulated N-cadherin. Statistical analyses between different cell groups were examined by Student's <i>t</i> test. ** represents for <i>p</i><0.01. g. Expression of Kindlin-2 in lymph node metastasized large cell lung cancer patient. A. Primary large cell lung cancer; B. Lymph node metastasized large cell lung cancer from the same patient.