Effect of vaccination with Δhsp70-II Leishmania mutant on development of a BALB/c CL model produced by mCherry+<i>L. major</i> strain.

<p>A) Top panel shows a non-infected animal (negative control) and the fluorescent signal emitted by hind footpads of animals infected with 2×10⁵ metacyclic mCherry+<i>L. major</i> promastigotes at different time points. Bottom panel shows the footpads of animals immunized by intravenous administration of 10⁷ Δhsp70-II metacyclic promastigotes per mouse four weeks before the challenge. Six mice in each group were imaged weekly. B) Parasite burdens in popliteal lymph nodes mean (LDAU/lymph node×10⁵) ± SEM of six animals. Statistical differences were observed between groups; *, <i>P</i><0.01 using de paired Student <i>t</i> test.