a<p>Underlined sequence indicates restriction site.</p>b<p>Bold sequence indicates optimized translation initiation sequence.</p>c<p>Orientation of primers: F, forward; R, reverse.</p
*<p>In section C, the nucleotide forward or reverse oligo are in bolded and the stem loop sequences ...
a<p>Restriction endonuclease sites incorporated in the oligonucleotide sequences are underlined.</p
a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the ...
<p><sup>a</sup>Underlined sequence indicates restriction site.</p><p><sup>b</sup>Bold sequence indic...
a<p>Underlines indicate the restriction sites.</p>b<p>Bold indicates the priming site.</p
<p>Note: Bold and underlined nucleotide sequence corresponds to the restriction enzyme sites (6 base...
<p><b>*</b> Underlined nucleotides denote enzyme restriction sites.</p><p>Oligonucleotide primers us...
a<p>Underlines indicate the restriction sites.</p>b<p>Bold indicates the priming site.</p>c<p>Bold a...
<p>Restriction sites inserted into the PCR product through the primers are underlined.</p
<p>Restriction sites are shown in italics. BglBrick prefix in forward (FW) primer and suffix in reve...
<p>*Restriction sites within the oligonucleotide sequences are printed in bold.</p><p>Oligonucleotid...
<p>Abbreviations: 7G, 7-glycine linker; F, forward primer; R, reverse primer. Restriction enzyme sit...
<p>Nucleotide sequence of primers used. Restriction sites are underlined; F = forward; R = rever...
a<p>The sequence in boldface type is the EcoRI restriction enzyme site.</p>b<p>The sequence in boldf...
<p>F and R indicate the forward and reverse directions, respectively. The underlined regions represe...
*<p>In section C, the nucleotide forward or reverse oligo are in bolded and the stem loop sequences ...
a<p>Restriction endonuclease sites incorporated in the oligonucleotide sequences are underlined.</p
a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the ...
<p><sup>a</sup>Underlined sequence indicates restriction site.</p><p><sup>b</sup>Bold sequence indic...
a<p>Underlines indicate the restriction sites.</p>b<p>Bold indicates the priming site.</p
<p>Note: Bold and underlined nucleotide sequence corresponds to the restriction enzyme sites (6 base...
<p><b>*</b> Underlined nucleotides denote enzyme restriction sites.</p><p>Oligonucleotide primers us...
a<p>Underlines indicate the restriction sites.</p>b<p>Bold indicates the priming site.</p>c<p>Bold a...
<p>Restriction sites inserted into the PCR product through the primers are underlined.</p
<p>Restriction sites are shown in italics. BglBrick prefix in forward (FW) primer and suffix in reve...
<p>*Restriction sites within the oligonucleotide sequences are printed in bold.</p><p>Oligonucleotid...
<p>Abbreviations: 7G, 7-glycine linker; F, forward primer; R, reverse primer. Restriction enzyme sit...
<p>Nucleotide sequence of primers used. Restriction sites are underlined; F = forward; R = rever...
a<p>The sequence in boldface type is the EcoRI restriction enzyme site.</p>b<p>The sequence in boldf...
<p>F and R indicate the forward and reverse directions, respectively. The underlined regions represe...
*<p>In section C, the nucleotide forward or reverse oligo are in bolded and the stem loop sequences ...
a<p>Restriction endonuclease sites incorporated in the oligonucleotide sequences are underlined.</p
a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the ...