A: Ex vivo IFNγ ELISpot responses in patients with acute DHF who developed shock and those who did not develop shock and also in healthy dengue seropositive donors and dengue seronegative donors.

<p>B: Spontaneously released IL-10 levels (pg/ml) in PBMC ELISpot supernatants in patients with shock and those without shock The mean spontaneous release of IL-10 from PBMC in patients with shock was 19.65 (SD±21.28 pg/ml), and the mean levels in those who did not develop shock was 11.28 (SD±24.5) pg/ml (p = 0.08). C: Spontaneously released TNFα levels (pg/ml) in PBMC ELISpot supernatants in patients with shock and those without shock. The mean spontaneous release of TNFα from PBMC ELISpot supernatants in patients with shock was 2193(SD±2094) pg/ml and the mean levels ino those who did not develop shock was721.4S9D±1921) pg/ml). p = 0.009. D: Correlation of spontaneously released TNFα levels (pg/ml) and IL-10 levels (pg/ml) in the PBMC ELISpot supernatants in patients with DHF. Spearmans r = 0.88, p<0.0001.